Open AccessLipopolysaccharides of chemolithotrophic bacteria Thiobacillus versutus and a related Thiobacillus species
Author(s) -
Yokota Akira,
Schlecht Siegfried,
Mayer Hubert
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02267.x
Subject(s) - thiobacillus , heptose , biochemistry , rhodobacter , bacteria , chemistry , glucosamine , biology , microbiology and biotechnology , genetics , mutant , gene
The lipopolysaccharides (LPSs) of two strains of Thiobacillus versutus and a Thiobacillus sp. strain were isolated and chemically analyzed. They contained neutral sugars, glucosamine, 2‐keto‐3‐deoxyoctonate (KDO), and phosphorus, but were devoid of heptose. The fatty acids were characterized as amide‐linked 3‐OH‐14:0 and 3‐oxo‐14:0, and ester‐linked 3‐OH‐10:0. The lipid A backbones contain glucosamine as the only amino sugar. Deoxycholate‐polyacrylamide gel electrophoresis (DOC‐PAGE) showed that the LPSs have R‐type character. The presence of amide‐linked 3‐OH‐14:0 and 3‐oxo‐14:0 and of ester‐linked 3‐OH‐10:0, the lack of heptose, and the R‐type character of LPSs, indicate a structure of LPSs of T. versutus and Thiobacillus sp. strains similar to those found for the phylogenetically related Rhodobacter species and Paracoccus denitrificans .