Open AccessUse of staphylococcal nuclease gene as DNA probe for Staphylococcus aureus
Author(s) -
Liebl W.,
Rosenstein R.,
Götz F.,
Schleifer K.H.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02264.x
Subject(s) - nuclease , dna , gene , staphylococcus aureus , micrococcal nuclease , biology , microbiology and biotechnology , base pair , hybridization probe , genetics , bacteria , nucleosome , histone
A 518‐base pair fragment of the cloned gene coding for the thermostable nuclease of Staphylococcus aureus strain Foggi was used as a DNA probe for hybridization to DNA of various staphylococci. The strains were tested for their ability to produce DNase. All strains of S. aureus produced heat‐stable DNase and their DNA reacted with the nuclease gene. Other staphylococci producing heat‐stable DNase did not react in a dot hybridization with the nuclease gene probe.