Open AccessIdentification and characterization of a novel β‐lactamase TLE‐2, encoded by plasmid pUK702
Author(s) -
Reid Alison J.,
Simpson I.N.,
Harper P.B.,
Amyes S.G.B.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02255.x
Subject(s) - plasmid , klebsiella pneumoniae , enzyme , strain (injury) , biology , substrate (aquarium) , microbiology and biotechnology , chemistry , biochemistry , gene , escherichia coli , ecology , anatomy
A novel plasmid‐mediated β‐lactamase has been identified in a Klebsiella pneumoniae strain, carried by a plasmid pUK702. The pUK702 plasmid encodes TEM‐1 and SHV‐1 β‐lactamase enzymes in addition to the novel enzyme. The novel enzyme has an M r of 19 000 and a p I of 6.5. The enzyme is TEM‐like in substrate profile and has been designated TLE‐2 (TEM‐like enzyme) on this basis. However, TLE‐2 can be distinguished from TEM‐1 and SHV‐1 in inhibition tests and failure to cross‐react in immunological tests.