Open AccessBacteriophage MB78 DNA synthesis is specifically inhibited by the chelating agent ethylene diamine tetraacetic acid
Author(s) -
Verma Mukesh,
Chakravorty Maharani
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02253.x
Subject(s) - chelation , bacteriophage , dna , egta , salmonella , ethylene diamine , dna synthesis , chemistry , microbiology and biotechnology , biochemistry , bacteria , biology , escherichia coli , nuclear chemistry , organic chemistry , calcium , gene , genetics
The growth of bacteriophage MB78, a virulent phage of Salmonella typhimurium is extremely sensitive to the chelating agent EDTA. Other chelating agents like EGTA, a specific chelator for Ca 2+ and orthophenanthroline which chelates Zn 2+ and Fe 2+ have no effect. EDTA stops phage MB78 DNA synthesis while synthesis of host DNA and other Salmonella phage DNA are not affected in presence of such low concentrations of EDTA. The present report indicates that some early phage function(s) and most probably the phage DNA synthesis are sensitive to EDTA which is probably due to chelation of Mg 2+ .