Open AccessNucleotide sequence analysis and comparison of the structural genes for Shiga‐like toxin I and Shiga‐like toxin II encoded by bacteriophages from Escherichia coli 933
Author(s) -
Jackson Matthew P.,
Neill Roger J.,
O'Brien Alison D.,
Holmes Randall K.,
Newland John W.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02252.x
Subject(s) - operon , escherichia coli , biology , nucleic acid sequence , bacteriophage , gene , shiga toxin , shiga like toxin , structural gene , genetics , toxin , sequence analysis , peptide sequence , sequence (biology) , microbiology and biotechnology
The nucleotide sequence of the Shiga‐like toxin type II (SLT‐II) structural genes cloned from bacteriophage 933W of the enterohemorrhagic Escherichia coli O157:H7 strain 933 was determined. This sequence was compared with the published sequence for the structural genes of the antigenically distinct Shiga‐like toxin type I (SLT‐I) encoded by bacteriophage 933J. The SLT‐I and SLT‐II structural genes shared 58% overall nucleotide and 56% amino acid sequence homologies. The A and B subunits of SLT‐I and SLT‐II were nearly identical in size and had similar secondary structures and hydropathy plots. The regulation proposed for the SLT‐II operon is similar to that previously proposed for SLT‐I.