Open AccessTransformation of a cloning vector, pUB110, into Bacillus anthracis
Author(s) -
Makino Souichi,
Sasakawa Chihiro,
Uchida Ikuo,
Terakado Nobuyuki,
Yoshikawa Masanosuke
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02239.x
Subject(s) - bacillus anthracis , transformation (genetics) , plasmid , kanamycin , cloning vector , cloning (programming) , microbiology and biotechnology , molecular cloning , vector (molecular biology) , chemistry , biology , bacteria , dna , genetics , recombinant dna , gene , antibiotics , peptide sequence , computer science , programming language
A kanamycin‐resistance plasmid, pUB110, was introduced into Bacillus anthracis by a newly devised method of transformation. The transformation frequency was ca. 1 × 10 3 cells/1 μ g DNA.