Open AccessCloning of a Vero toxin (VT1, Shiga‐like toxin I) gene from a VT1‐converting phage isolated from Escherichia coli O157:H7
Author(s) -
Kurazono Hisao,
Sasakawa Chihiro,
Yoshikawa Masanosuke,
Takeda Yoshifumi
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02235.x
Subject(s) - ecori , escherichia coli , shiga like toxin , microbiology and biotechnology , shiga toxin , toxin , biology , strain (injury) , plasmid , chemistry , dna , gene , genetics , anatomy
Vero toxin (VT1, Shiga‐like toxin I)‐converting phages were induced with UV light from Escherichia coli O157:H7 strain 83–1386. A non‐toxigenic E. coli C600 strain, lysogenized with a toxin‐converting phage (86‐02), produced VT1. A phage solution was prepared from the lysogenized E. coli C600 (86‐02) strain and the phage DNA was prepared. Eco RI‐fragments of the phage DNA were ligated with Eco RI‐digested pBR325ΔTc and it was transformed into E. coli strain MC1061. Transformants with VT1 production commonly contained a 5.1‐kb Eco RI‐fragment. The restriction map of the Eco RI‐fragment was prepared and it was found that a 2.1‐kb Bam HI‐ Bgl II fragment encoded VT1 production.