Open AccessIsolation of the gene encoding the major endoglucanase of Erwinia chrysanthemi . Homology between cel genes of two strains of Erwinia chrysanthemi
Author(s) -
Boyer MariHélène,
Cami Brigitte,
Kotoujansky Alain,
Chambost JeanPierre,
Frixon Chantal,
Cattanéo Jeanne
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02225.x
Subject(s) - homology (biology) , erwinia , gene , biology , microbiology and biotechnology , isolation (microbiology) , genetics
The structural gene encoding the major endoglucanase (EGZ) of Erwinia chrysanthemi strain 3665 has been isolated by hybridization using a DNA fragment of E. chrysanthemi strain 3937 previously cloned in the λL47‐1 vector and expressing an endoglucanase activity. Furthermore, genes homologous to celZ and celY of strain 3665 were also isolated and subcloned from strain 3937 into Escherichia coli . They were shown to encode enzymes similar to EGZ and EGY of strain 3665.