Open AccessCalcium enhances Salmonella typhimurium invasion of HeLa cells
Author(s) -
Niesel David W.,
Peterson Johnny W.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02215.x
Subject(s) - infectivity , hela , calcium , salmonella , in vitro , microbiology and biotechnology , chemistry , biology , cell , cell culture , biochemistry , bacteria , virology , virus , genetics , organic chemistry
Salmonella typhimurium cultured with physiological levels of calcium are significantly enhanced in their ability to penetrate HeLa cells in vitro. Increased infectivity was not observed in magnesium‐supplemented media, but was demonstrated in calcium‐supplemented minimal defined or calcium‐supplemented cation‐deficient media. Invasion enhancement was observed for a number of S. typhimurium strains and ranged from 28–390% over calcium‐deficient controls. Enhanced HeLa cell infectivity was not dependent on the presence of an autonomous 60‐MDa plasmid.