Open AccessStability of nucleic acids in immobilized and desiccated Nostoc commune UTEX584 (Cyanobacteria)
Author(s) -
Stulp Ben K.,
Potts Malcolm
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02204.x
Subject(s) - nucleic acid , cyanobacteria , desiccation , uracil , dna , nostoc , microscale chemistry , biochemistry , biology , chemistry , enzyme , botany , bacteria , genetics , mathematics education , mathematics
Two microscale methods were developed to isolate and analyze the [2‐ 14 C]uracil‐labeled nucleic acids of immobilized, dried cells of the desiccation‐tolerant cyanobacterium Nostoc commune UTEX584. The incidence of single‐strand breaks (‘nicks’) in DNA of light‐desiccated, but not dark‐desiccated cells, was demonstrated by the use of DNA modification enzymes.