Open AccessNucleotide sequence of the sfaA gene coding for the S‐fimbrial protein subunit of Escherichia coli
Author(s) -
Schmoll Thomas,
Hacker Jörg,
Goebel Werner
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02202.x
Subject(s) - fimbria , escherichia coli , homology (biology) , nucleic acid sequence , gene , protein subunit , biology , coding region , peptide sequence , amino acid , microbiology and biotechnology , genetics , biochemistry
The sfaA gene of the uropathogenic Escherichia coli O6 strain 536, which is responsible for the determination of the S fimbrial protein subunit, was sequenced. The structural gene codes for a polypeptide of 180 amino acids including a 24‐residue N‐terminal signal sequence. A size of 15.95 kDa was calculated for the processed SfaA protein. The nucleotide and deduced amino acid sequences show significant homology to those of the F1C fimbria and, to a lesser extent, of the mannose‐sensitive hemagglutinating fimbria (FimA, PilA). Only week homology to P fimbriae subunits (F7 2 , Pap) was found.