Open AccessTransfer of broad host range plasmids to the type I obligate methanotroph Methylomonas albus
Author(s) -
McPheat William L.,
Mann Nicholas H.,
Dalton Howard
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02193.x
Subject(s) - plasmid , kanamycin , escherichia coli , microbiology and biotechnology , streptomyces albus , biology , transposable element , bacteriophage , bacteria , genetics , mutant , dna , antibiotics , gene , streptomyces
Plasmids RP4 (IncP) and R300B (IncQ) (mobilized by RP4) were transferred to Methylomonas albus from Escherichia coli donors at frequencies of 2 × 10 −3 and 1−10 −3 per donor. BY contrast, plasmids pS‐a (IncW) and the IncP plasmids R68.45, R751 and derivatives carrying bacteriophage Mu and/or transposons were transferred to M. albus from E. coli at frequencies of 10 −7 –10 −8 per donor. M. albus transconjugants carrying R68.45, R751 and their derivatives were isolated and used as donors in matings with either other strains of M. albus or E. coli . Plasmids carrying bacteriophage Mu were transferred to E. coli at a frequency of 10 −5 –10 −6 per donor and 10 −2 –10 −3 per donor for those not carrying Mu. Transfer to M. albus recipients was at a frequency of 10 −3 –10 −4 per donor regardless of the presence or absence of Mu, indicating the absence of a suicide effect. Plasmids carrying Tn5 were found in M. albus to determine simultaneous kanamycin (Km) and streptomycin (Sm) resistance.