Open AccessCloning of the sacS gene encoding a positive regulator of the sucrose regulon in Bacillus subtilis
Author(s) -
Débarbouillé Michel,
Kunst Frank,
Klier André,
Rapoport Georges
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02184.x
Subject(s) - levansucrase , bacillus subtilis , regulon , plasmid , biology , gene , mutant , escherichia coli , genetics , cloning (programming) , molecular cloning , microbiology and biotechnology , allele , recombinant dna , bacteria , gene expression , computer science , programming language
The sacS gene controls the expression of 2 saccharolytic enzymes in Bacillus subtilis (sucrase and levansucrase). This paper describes a recombinant plasmid containing a mutant allele, sacS c . The plasmid was isolated from a B. subtilis DNA bank established in Escherichia coli . Moreover, it was shown that the sacS c allele, placed on a high‐copy plasmid, is dominant over the wild‐type chromosomal sacS + allele. This result strongly suggests that the sacS gene encodes a positive regulatory protein.