Open AccessCarbon monoxide metabolism in cell suspensions of Peptostreptococcus productus strain Marburg
Author(s) -
Ma Kesen,
Siemon Sabine,
Diekert Gabriele
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02174.x
Subject(s) - peptostreptococcus , strain (injury) , carbon monoxide , bacteria , chemistry , substrate (aquarium) , organism , metabolism , biochemistry , microbiology and biotechnology , biology , anaerobic bacteria , catalysis , anatomy , ecology , genetics , paleontology
Peptostreptococcus productus strain Marburg is able to grow on CO as the sole energy source and converts this substrate to acetate and CO 2 according to the following equation: 4 CO + 2 H 2 O → 1 acetate − + 1 H + + 2 CO 2 In cell suspensions of the organism the apparent K m value for CO conversion to acetate was about 270 μM (∼ 25% in the gas phase at 140 kPa). Cells incubated in the presence of 14 CO and unlabelled CO 2 incorporated CO specifically into the carboxyl group of acetate like other acetogenic bacteria tested. However, in contrast to experiments performed with other acetogens, 100% of the carboxyl group formed was derived from CO rather than from CO 2 indicating that ‘free’ CO rather than a bound carbonyl formed from CO 2 may be the precursor for the synthesis of the carboxyl group of acetate in this organism.