Open AccessProtein synthesis in cell‐free extracts of Dictyostelium discoideum
Author(s) -
Ntamere Alphonsus S.,
Barclay Stephen L.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02136.x
Subject(s) - dictyostelium discoideum , eukaryote , methionine , protein biosynthesis , in vitro , messenger rna , endogeny , biochemistry , translation (biology) , cell free system , heat shock protein , microbiology and biotechnology , chemistry , dictyostelium , biology , amino acid , gene , genome
We have developed an in vitro translation system for the lower eukaryote Dictyostelium discoideum . Active extracts using endogenous mRNA support protein synthesis with optimal Mg 2+ and K + concentrations of 5 mM and 120 mM, respectively. [ 35 S]Methionine incorporation is linear for more than 2 h. Polypeptides synthesized from endogenous mRNA have sizes ranging from less than 20 to over 100 kDa. Heat‐shock proteins are synthesized in vitro in extracts prepared from heat‐shocked cells. Possible uses of this system for study of translational control during growth and differentiation are discussed.