Open AccessInhibition of epithelial cell invasion in vitro by Yersinia enterocolitica with anticellular antibody
Author(s) -
Schiemann Donald A.,
Crane Marlys R.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02134.x
Subject(s) - yersinia enterocolitica , antiserum , antibody , microbiology and biotechnology , ouchterlony double immunodiffusion , hela , vero cell , precipitin , in vitro , yersinia , cell culture , serotype , chemistry , biology , immunodiffusion , bacteria , biochemistry , immunology , genetics
An antiserum prepared in rabbits against Henle 407 intestinal epithelial cells inhibited invasion of Henle and HeLa cells by three serotypes of Yersinia enterocolitica but was inactive with Vero cells which are of monkey rather than human origin. Purified immunoglobulins and IgG antibody were also inhibitory but univalent Fab fragments were inactive. A single precipitin line of identity was observed with Henle and HeLa cell extracts in an Ouchterlony immunodiffusion assay. The results provide additional evidence that bacterial invasion is dependent upon phagocytic activity of the epithelial cells.