Open AccessInvolvement of transposable elements in the formation of hybrid phages between bacteriophage P1 and the R plasmid NR1
Author(s) -
Iida Shigeru,
Meyer Jürg,
Mise Katsutoshi,
Arber Werner
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02107.x
Subject(s) - transposable element , replicon , plasmid , biology , genetics , dna transposable elements , transposition (logic) , homologous recombination , insertion sequence , genome , tn3 transposon , recombination , bacteriophage , dna , gene , escherichia coli , linguistics , philosophy
Homologous recombination between IS 1 elements present on both replicons, P1 and NR1, resulted in P1‐NR1 cointegrates and P1‐RTF and P1‐r‐det phages. Cointegration between P1 and NR1‐B, and NR1 derivative with multiple DNA rearrangements including insertion of the transposable element γδ, was also mediated by reciprocal recombination in IS 1 sequences. However, all 4 hybrids studied carried deletions promoted by γδ residing on NR1‐B. Further IS 1 ‐mediated deletions on the hybrid genomes resulted in plaque‐forming P1Cm phages.