Open AccessCytotoxic activity of Clostridium butyricum supernatants induced by butyrate
Author(s) -
Popoff Michel R.,
JolivetReynaud Colette,
Carlier Jean P.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02104.x
Subject(s) - clostridium butyricum , vero cell , butyric acid , microbiology and biotechnology , cytotoxic t cell , butyrate , clostridium , titer , cell culture , biology , chemistry , fermentation , bacteria , virology , biochemistry , in vitro , virus , genetics
Culture filtrates of 37 Clostridium butyricum strains induced morphological changes in Vero cell monolayers and growth inhibition of MRC5 fibroblasts and Vero cells. Partial purification of culture filtrate showed that the cytotoxic factor was related to butyric acid which is one of the main fermentation end products of C. butyricum . Cytotoxic titer was dependent upon butyric acid concentration. No difference was found between the amounts of butyric acid produced by different strains of C. butyricum isolated from either healthy newborns or newborns with neonatal necrotizing enterocolitis. The role of butyric acid as a potential virulence factor is discussed.