Open AccessMutants of Schizosaccharomyces pombe defective in glycerol catabolism
Author(s) -
Kong Y.C.,
Marshall J.H.,
May J.W.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02100.x
Subject(s) - dihydroxyacetone , glycerol kinase , glycerol , dihydroxyacetone phosphate , schizosaccharomyces pombe , biochemistry , glycerol 3 phosphate dehydrogenase , mutant , catabolism , schizosaccharomyces , dehydrogenase , nad+ kinase , biology , oxidoreductase , dhap , chemistry , enzyme , gene
Mutants of Schizosaccharomyces pombe unable to grow or growing very slowly on glycerol have been isolated. Some, which could grow on dihydroxyacetone, lacked, or in one mutant possessed reduced amounts of, glycerol dehydrogenase (glycerol:NAD + 2‐oxidoreductase); others could not grow on dihydroxyacetone and lacked dihydroxyacetone kinase. Spontaneous revertants able to grow on glycerol had regained these enzymes. These results provide evidence that glycerol catabolism in S. pombe proceeds via initial dehydrogenation of glycerol followed by phosphorylation of dihydroxyacetone. There is evidence that dihydroxyacetone can be converted to a toxic product.