Open AccessChanges in phosphorus magnetic resonance spectra during the cell cycle of phosphorus limited phased culture of Candida utilis
Author(s) -
Dawson P.S.S.,
MacDonald J.C.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02097.x
Subject(s) - polyphosphate , phosphate , phosphorus , cell cycle , biology , cell culture , chemistry , biochemistry , cell , organic chemistry , genetics
Cell extracts, serially obtained from Candida utilis grown in continuous (synchrony) culture under phosphate limitation during an 8‐h cycle and examined by NMR spectroscopy, revealed changes in polyphosphate content during the cycle period: other phosphorus containing components showed relatively little change. Initially zero, the polyphosphate content increased rapidly to a maximum after 30 min that coincided with exhaustion of phosphate from the culture, and then decreased slowly back to zero at the end of the cycle. The results suggest that polyphosphate, usually considered to function as a reserve material, actively participates during the cell cycle.