Open AccessCharacterization and biological effects of the Pasteurella multocida toxin
Author(s) -
Foged N.T.,
Pedersen K.B.,
Elling F.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02095.x
Subject(s) - pasteurella multocida , toxin , microbiology and biotechnology , resorption , nasal administration , bone resorption , chemistry , pasteurella , biological activity , polyacrylamide gel electrophoresis , bacteria , gel electrophoresis , biology , in vitro , biochemistry , immunology , endocrinology , enzyme , genetics
A proteinaceous substance produced by a toxigenic type D strain of Pasteurella multocida was purified by anion exchange chromatography and preparative polyacrylamide gel electrophoresis (PAGE). The protein, the P. multocida toxin (PMT), had a molecular weight of 143 kDa (SDS‐PAGE) and had the same biological properties as crude extracts from toxigenic strains of P. multocida i.e., dermonecrotic effect in guinea pigs, cytopathic effect in embryonic bovine lung cells, and a lethal effect to mice. PMT induced bone resorption of the turbinates and other bone structures in the nasal cavity, when it was instilled intranasally to newborn rats and piglets. Histological and ultrastructural examinations showed that the bone resorption was brought about by enhanced osteoclastic activity and impaired osteoblastic bone formation. Inflammatory reactions were not observed. PMT is supposed to be the causative substance of atrophic rhinitis in pigs.