Open AccessAnabolic role of l ‐malic acid in Saccharomyces cerevisiae in anaerobiosis during alcoholic fermentation
Author(s) -
Salmon J.M.,
Vezinhet F.,
Barre P.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02075.x
Subject(s) - biochemistry , citric acid cycle , anabolism , pyruvate carboxylase , malate dehydrogenase , malic acid , malic enzyme , fructose , fermentation , glycolysis , ethanol fermentation , chemistry , biology , dehydrogenase , metabolism , enzyme , citric acid
In anaerobiosis, pyruvate carboxylase activity can be greatly reduced by limiting the availability of biotin to yeast cells. In these conditions an incorporation of carbon from exogenous l ‐malic acid can be observed. The main pathway used for this anabolic utilization goes through malate dehydrogenase and the oxidative reactions of the tricarboxylic acid cycle. This anabolic function of l ‐malic acid is strongly inhibited by a residual glucose concentration in the fermenter. This inhibition may be due, in this condition, to a higher intracellular concentration of some negative effectors of malate dehydrogenase such as: 1,6‐fructose diphosphate, glyceraldehyde 3‐phosphate, ATP and ADP.