Open AccessShotgun cloning in Streptococcus lactis
Author(s) -
Santos Alda Luiza Loureiro,
Chopin Alain
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02074.x
Subject(s) - shotgun sequencing , cloning (programming) , biology , plasmid , shotgun , genetics , transformation (genetics) , cloning vector , transposable element , transposition (logic) , molecular cloning , clone (java method) , dna , microbiology and biotechnology , gene , dna sequencing , genome , peptide sequence , computer science , programming language , linguistics , philosophy
Using plasmid vector pIL204 and a recently described high‐efficiency transformation protocol, we performed a direct shotgun cloning experiment in Streptococcus lactis . The cloned DNA was a 22 kilobases fragment of the S. lactis chromosome previously labelled by insertion of transposon Tn 916 encoding tetracycline resistance. An interesting feature of the present shotgun cloning system is its ability to clone large‐size DNA fragments.