Open AccessExpression of the E. coli lexA + gene in Erwinia carotovora subsp. carotovora and its effect on production of pectin lyase and carotovoricin *
Author(s) -
McEvoy James L.,
Thurn Kerry K.,
Chatterjee Arun K.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02073.x
Subject(s) - erwinia , pectin lyase , repressor lexa , gene , escherichia coli , biology , bacteria , microbiology and biotechnology , chemistry , enterobacteriaceae , gene expression , enzyme , biochemistry , genetics , repressor , pectinase
In Erwinia carotovora subsp. carotovora, recA mediates various SOS functions as well as the DNA damage‐induced synthesis of pectin lyase (PNL) and carotovoricin (CTV). To further characterize this system a plasmid carrying the E. coli lexA + gene was introduced into E. carotovora subsp. carotovora strain 71. The construct was sensitive to ultraviolet light and lost the ability to synthesize two nalidixic acid‐inducible proteins, one with an electrophoretic mobility similar to that of the E. coli RecA. Basal and nalidixic acid‐induced levels of PNL and CTV were increased several‐fold in the construct, suggesting that an interaction occurs between LexA and some regulatory element(s) of pnl and ctv .