Open AccessGenomic distribution of repeated and single copy DNA sequences in Penicillium funiculosum *
Author(s) -
Sahasrabudhe N.A.,
Sainani M.N.,
Gupta V.S.,
Ranjekar P.K.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02046.x
Subject(s) - dna , biology , genetics , genomic dna , repeated sequence , nucleotide , dna sequencing , microbiology and biotechnology , agarose gel electrophoresis , genome , gene
Study of the reassociation kinetics of Penicillium funiculosum DNA (fragment size > 20 kb) revealed an interspersion of repeated and single‐copy DNA sequences. The fragment length of interspersed single‐copy DNA was estimated to be more than 9 kb from a curve relating the fraction of DNA binding to hydroxyapatite as a function of DNA fragment length. The length of interspersed short and long repeated nucleotide sequences was determined to be 0.55 kb and > 20 kb, respectively, by agarose gel electrophoresis. The genomic organization of P. funiculosum is thus significantly different from that in other fungi.