Open AccessA direct demonstration of hydrogen cycling by Desulfovibrio vulgaris employing membrane‐inlet mass spectrometry
Author(s) -
Peck Harry D.,
LeGall Jean,
Lespinat Paul A.,
Berlier Yves,
Fauque Guy
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02042.x
Subject(s) - desulfovibrio vulgaris , desulfovibrio , sulfate , mass spectrometry , chemistry , sulfate reducing bacteria , hydrogen , bacteria , membrane , chromatography , biochemistry , environmental chemistry , biology , organic chemistry , genetics
Employing membrane‐inlet mass spectrometry, direct evidence is presented that substrate amounts of hydrogen are simultaneously produced and consumed during the metabolism of pyruvate plus sulfate by washed intact cells of Desulfovibrio vulgaris (Hildenborough). The results clearly demonstrate that hydrogen cycling is an important bioenergetic mechanism for this sulfate‐reducing bacterium when growing on pyruvate plus sulfate.