Open AccessThe use of gel‐stabilized gradient plates to map the responses of microorganisms to three or four environmental factors varied simultaneously
Author(s) -
Wimpenny Julian,
Waters Paul
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02036.x
Subject(s) - microorganism , wedge (geometry) , nitrate , concentration gradient , chemistry , range (aeronautics) , dimension (graph theory) , bacteria , environmental chemistry , mineralogy , environmental science , geology , materials science , geometry , mathematics , composite material , organic chemistry , paleontology , pure mathematics
The Szybalski wedge plate technique has been used to map the responses of bacteria to up to 4 simultaneously varying environmental factors. 2‐dimensional NaCl‐pH gradient plates were used throughout. Sets of the latter were incubated at a range of temperatures to provide a third dimension. A fourth variable, nitrate concentration, was investigated by incorporating it homogeneously in sets of NaCl‐pH gradient plates.