Open AccessPurification and characterization of chromosomal streptomycin adenylyltransferase from derivatives of Bacillus subtilis Marburg 168
Author(s) -
Kono Megumi,
Ohmiya Keiichi,
Kanda Toshihisa,
Noguchi Norihisa,
O'hara Koji
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02029.x
Subject(s) - bacillus subtilis , isoelectric point , enzyme , streptomycin , biochemistry , bacillaceae , chemistry , biology , bacillales , microbiology and biotechnology , bacteria , genetics , antibiotics
Chromosomal streptomycin(Sm) adenylyltransferase was detected in Sm‐susceptible derivatives of Bacillus subtilis Marburg 168. The enzyme was purified from one of the strains and its enzymological properties were investigated. The isoelectric point and m r of the enzyme were 6.0 and 35 000, respectively. The optimum pH was 8.0 and the optimum temperature for the inactivation of Sm was 35°C.