Open AccessHydrogen metabolism during methanogenesis from acetate by Methanosarcina barkeri
Author(s) -
Krzycki J.A.,
Morgan J.B.,
Conrad R.,
Zeikus J.G.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02024.x
Subject(s) - methanogenesis , methanosarcina barkeri , hydrogenase , methanosarcina , chemistry , carbon monoxide dehydrogenase , chloroform , hydrogen , ethyl acetate , hydrogen production , biochemistry , inorganic chemistry , methane , methanomicrobiales , carbon monoxide , nuclear chemistry , organic chemistry , catalysis
A tritium exchange assay and a sensitive gas chromatographic technique were used to demonstrate that hydrogenase was active and that hydrogen was produced by Methanosarcina barkeri strain MS grown on acetate. Both methane and hydrogen production rates were dependent on the concentration of acetate in the medium. H 2 was produced at 0.5–2% of the rate of CH 4 formation. Chloroform and potassium cyanide, inhibitors of methanogenesis from acetate, inhibited H 2 production but not hydrogenase activity. The addition of hydrogen gas to cell suspensions did not inhibit CH 4 or carbon dioxide production from the methyl group of acetate. H 2 production appears to be linked to several intracellular redox processes which follow the cleavage of acetate.