Open AccessAnalysis of peptide uptake in Pseudomonas aeruginosa : A fluorescamine labeling procedure
Author(s) -
Hulen Christian,
Goffic François
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb01991.x
Subject(s) - tripeptide , fluorescamine , peptide , pseudomonas aeruginosa , chemistry , hydrolysis , chromatography , oligopeptide , intracellular , biochemistry , bacteria , biology , physics , quantum mechanics , fluorescence , genetics
Intracellular products of peptide transport were analyzed after thin layer chromatography separation by a fluorescamine labeling procedure. Dipeptides penetrated Pseudomonas aeruginosa via 2 transport systems, one of high affinity with K T values from 1–2 μM, and another one of low affinity ( K T about 30 μM). A single transport system assumed tripeptide uptake with K T varying from 10–30 μM, depending on the tripeptide used. Peptides entering the cells were rapidly hydrolyzed into their constitutive amino acids.