Open AccessMonoclonal antibodies detecting novel structures in the core region of Salmonella minnesota lipopolysaccharide
Author(s) -
Appelmelk B.J.,
Verweijvan Vught A.M.J.J.,
Maaskant J.J.,
Schouten W.F.,
Thijs L.G.,
MacLaren D.M.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb01985.x
Subject(s) - monoclonal antibody , lipopolysaccharide , epitope , immunization , antibody , salmonella , microbiology and biotechnology , biology , bacteria , chemistry , virology , immunology , genetics
Following immunization of mice with Salmonella minnesota strain R595, monoclonal antibodies to R595 lipopolysaccharide were prepared. These belonged to various (sub)classes: IgG2a, IgG2b, IgG3 and IgM. Ig2a clones were obtained only when acetone‐killed bacteria were used for immunization. Long‐course immunizations yielded more clones than short courses. The reactivity of the monoclonal antibodies towards a panel of S. minnesota rough strain lipopolysaccharides (Ra to Re chemotype) was determined. One group of the antibodies reacted with Re and Rb2 lipopolysaccharide only, a second group reacted with Rc, Rd1 and Re, and a third group reacted with Re, Rb2 and Rd1 only. Thus, these monoclonal antibodies recognized epitopes whose presence cannot be explained by our current knowledge of the covalent structure of the S. minnesota core region.