Open AccessGenetic evidence that the outer membrane protein YOP1 of Yersinia enterocolitica mediates adherence and phagocytosis resistance to human epithelial cells
Author(s) -
Heesemann Jürgen,
Grüter Ludwig
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb01978.x
Subject(s) - yersinia enterocolitica , phagocytosis , microbiology and biotechnology , plasmid , bacterial outer membrane , biology , serotype , strain (injury) , recombinant dna , escherichia coli , bacteria , dna , gene , genetics , anatomy
We studied the role of Yersinia enterocolitica outer membrane protein YOP1 in adherence and phagocytosis resistance to the human epithelial cell line, HEp‐2. A 5.2‐kb DNA fragment of the plasmid of Y. enterocolitica, serotype O : 9, which encodes a 225‐kDa outer membrane protein (YOP1), was cloned into the mobilisable vector pRK290B. After introduction of the recombinant plasmid into a non‐adherent Y. enterocolitica strain of serotype O : 5, the resulting transconjugant acquired adherence and phagocytosis resistance properties to HEp‐2 cells. Furthermore, the plasmidless Y. enterocolitica strain of serotype O : 9 which is rapidly phagocytosed by HEp‐2 cells also became phagocytosis‐resistant after receipt of the YOP1‐encoding plasmid. These results indicate that YOP1 mediates cell adherence and phagocytosis resistance to HEp‐2 cells.