Open AccessEnzyme immunoassay for the detection of antibody to Pseudomonas pseudomallei exotoxin in mice
Author(s) -
Ismail G.,
Embi M.Noor,
Omar O.,
Razak N.,
Allen J.C.,
Smith C.J.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb01976.x
Subject(s) - melioidosis , exotoxin , burkholderia pseudomallei , immunoassay , pseudomonas exotoxin , microbiology and biotechnology , antibody , antitoxin , biology , toxin , pseudomonas , enzyme , virology , bacteria , pseudomonas aeruginosa , immunology , biochemistry , genetics
An enzyme immunoassay (enzyme‐linked immunosorbent assay; ELISA) to detect mouse antibody to exotoxin of Pseudomonas pseudomallei was developed in which exotoxin preparations were used to coat the solid phase. The specificity of the assay was supported by inhibition assays with the toxin preparations. Sera from immunised mice and supernatants from antitoxin‐producing hybridoma cells were tested by this technique, and the ELISA described appears to provide a sensitive, specific, and practical method for the determination of (a) antibody to exotoxin, and (b) concentration of exotoxin. Both systems may prove invaluable in the diagnosis of melioidosis and in epidemiological studies of melioidosis in endemic areas.