Open AccessMonoclonal antibodies to the surface antigens of Pseudomonas aeruginosa
Author(s) -
Gaston Michael A.,
Vale Teresa A.,
Wright Bruce,
Cox Peter,
Pitt Tyrone L.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01824.x
Subject(s) - serotype , monoclonal antibody , epitope , antibody , microbiology and biotechnology , pseudomonas aeruginosa , antigen , pseudomonadaceae , pseudomonas fluorescens , bacterial outer membrane , biology , virology , lipopolysaccharide , pseudomonas , pseudomonas putida , bacteria , escherichia coli , immunology , biochemistry , gene , genetics
A panel of 48 monoclonal antibodies was prepared against 8 O‐serotype strains of Pseudomonas aeruginosa , and 43 of the antibodies reacted specifically with whole cells of the vaccine strain in an enzyme‐linked immunosorbent assay (ELISA). 4 antibodies showed varying degrees of reactivity for more than one of the serotype strains, and one antibody bound to all of the serotype strains as well as strains of Pseudomonas putida and Pseudomonas fluorescens . The epitopes recognised by these antibodies were characterised by immunoblotting and the serotype‐specific antibodies reacted only with lipopolysaccharide (LPS) of the vaccine strain. The antibodies that bound to more than one serotype strain were specific for outer‐membrane proteins common to the serotype strains. The antibody that cross‐reacted with all strains of P. aeruginosa apparently recognised an antigen associated with the core or lipid A components of LPS.