Open AccessCalmodulin activity in yeast and mycelial phases of Ceratocystis ulmi
Author(s) -
Muthukumar Ganapathy,
Luby Maureen T.,
Nickerson Kenneth W.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01815.x
Subject(s) - calmodulin , trifluoperazine , ceratocystis , dibucaine , calcium , mycelium , yeast , biology , phosphodiesterase , biochemistry , botany , chemistry , enzyme , fungus , organic chemistry
The yeast and mycelial phases of Ceratocystis ulmi contained roughly equivalent levels of calmodulin activity as determined by their ability to stimulate calmodulin‐deficient bovine brain cAMP phosphodiesterase. This stimulation was calcium‐dependent and could be inhibited by either dibucaine or trifluoperazine. Also, the concentration of dibucaine necessary to achieve the mycelium‐to‐yeast morphological conversion was found to be 3‐fold greater in the presence of exogenous calcium. A model is presented in which only 30% of the cellular calmodulin need be complexed with calcium ions for mycelial development.