Open AccessLow copy number plasmid vectors for gene cloning and for monitoring gene expression
Author(s) -
Reuse Hilde,
Touati Eliette,
Glaser Philippe,
Danchin Antoine
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01792.x
Subject(s) - plasmid , cloning (programming) , gene , genetics , biology , gene expression , molecular cloning , vector (molecular biology) , computational biology , microbiology and biotechnology , recombinant dna , computer science , programming language
Derivatives of an IncW incompatibility plasmid with a low copy number are described which can be used for gene cloning or for analysing gene expression in conditions similar to those found in the host chromosome. Gene expression can be monitored after construction of operon or protein fusions with the lacZYA operon and measurement in Escherichia coli of the β‐galactosidase activity.