Open AccessSite‐specific recombination events mediated by the DNA invertase Cin of bacteriophage P1 during transformation
Author(s) -
Iida Shigeru
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01790.x
Subject(s) - plasmid , bacteriophage , recombinase , transformation (genetics) , recombination , dna , gene , biology , site specific recombination , cre recombinase , genetics , microbiology and biotechnology , transgene , escherichia coli , genetically modified mouse
Bacteriophage P1 encodes the site‐specific recombinase Cin which promotes inversion of the C segment, thus controlling the P1 host range. Cin can also mediate inefficient inversion between the normal crossover site cixL and a quasi‐crossover site cixQ 1 in inverted orientation. Inversion between cixL and cixQ 1 occurs more frequently in a short period of time after transformation with a plasmid carrying the cin gene, cixL and cixQ 1 than in an established transformant of the plasmid. This is also the case for Cin‐mediated deletion on a plasmid containing the cin gene and directly repeated cix sites.