Open AccessRole of a small plasmid in the modification of carbon monoxide dehydrogenase in Pseudomonas carboxydovorans
Author(s) -
Kwon M.O.,
Chung I.K.,
Kim Y.M.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01777.x
Subject(s) - plasmid , pseudomonas , protein subunit , carbon monoxide dehydrogenase , microbiology and biotechnology , enzyme , protease , strain (injury) , chemistry , transformation (genetics) , biochemistry , biology , gene , carbon monoxide , bacteria , catalysis , genetics , anatomy
Soluble fractions prepared from cells of Pseudomonas carboxydovorans bearing a small plasmid (1.76 × 10 6 ) exhibited proteolytic activity on the β‐subunit of CO dehydrogenase (CO‐DH) in plasmid‐cured cells of the same strain, implying that the plasmid carries gene(s) for processing the β subunit of the enzyme at the post‐translational level. The protease was found to be a constitutive enzyme. It did not hydrolyze the β subunit of CO‐DH in Pseudomonas carboxydohydrogena . Analysis of CO‐DH after transformation of the cured cells with the small plasmid confirmed that the plasmid plays a role in the modification of the β subunit of CO‐DH in P. carboxydovorans .