Open Accessp ‐Cresol biodegradation under denitrifying conditions: isolation of a bacterial coculture
Author(s) -
Bossert I.D.,
Rivera M.D.,
Young L.Y.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01743.x
Subject(s) - denitrifying bacteria , biodegradation , nitrate , chemistry , microorganism , electron acceptor , anaerobic exercise , environmental chemistry , food science , denitrification , metabolism , microbiology and biotechnology , p cresol , bacteria , biochemistry , biology , nitrogen , organic chemistry , physiology , genetics
From polluted river sediment, two bacterial species were isolated which utilized p ‐cresol as the sole source of carbon when grown in coculture under nitrate‐reducing conditions. One species, PC‐07, metabolized p ‐cresol (pCr) anaerobically to p ‐hydroxybenzoate (pOHB), which in turn was further metabolized by the second isolate, PB‐04. The PC‐07 isolate was unable to degrade and utilize pOHB, and PB‐04 was unable to utilize pCr, thereby demonstrating a syntrophic relationship for pCr utilization under anaerobic conditions. Nitrate served as external electron acceptor for both microorganisms under anaerobic conditions and was reduced via NO 2 − and N 2 O to N 2 . pCr, therefore, appears to be metabolized to ring fission products via the formation of pOHB under nitrate reducing conditions, with the metabolism being mediated by a 2‐member microbiol food chain.