Open AccessSurvival of Pseudomonas fluorescens and Bacillus subtilis introduced into two soils of different texture in field microplots
Author(s) -
Elsas J.D.,
Dijkstra A.F.,
Govaert J.M.,
Veen J.A.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01724.x
Subject(s) - pseudomonas fluorescens , loam , rhizosphere , soil water , bacillus subtilis , biology , soil texture , agronomy , pseudomonas , spore , soil classification , horticulture , bacteria , botany , ecology , genetics
Antibiotic‐resistant strains of Pseudomonas fluorescens and Bacillus subtilis , produced by transposon Tn5 mutagenesis and transformation with plasmid pFT30, respectively, were characterized. Both strains grew at a rate comparable to that of the wild‐type strains, and the antibiotic resistance remained stable for over 50 generations without selective pressure. During the growing season, the survival of these strains was studied in two soils of different texture cropped with wheat. The B. subtilis populations declined rapidly in both soils and then stabilized at the levels of added spores. P. fluorescens showed a slow, steady decline in both soils; survival was better in the finer‐textured soil, a silt loam, than in the coarser loamy sand. For both bacteria, some translocation to deeper soil layers was observed. No significant rhizosphere effects were detected in either of the two soils.