Open AccessIntracellular localization of the hydrogenase in Desulfotomaculum orientis
Author(s) -
Cypionka Heribert,
Dilling Waltraud
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01705.x
Subject(s) - hydrogenase , intracellular , chemistry , methylene blue , centrifugation , nad+ kinase , electron acceptor , biochemistry , bacteria , enzyme , biology , catalysis , photocatalysis , genetics
Cell extracts of Desulfotomaculum orientis , grown with H 2 plus sulfate as sole energy source, revealed hydrogenase activities between 0.3 and 2 μmol H 2 per min and mg protein when methyl viologen was used as electron acceptor. With benzyl viologen, methylene blue, FAD or FMN, lower activities were found; NAD was not reduced. The hydrogenase activity was strongly inhibited by CuCl 2 ; however, copper inhibition was not observed with whole cells, indicating that the hydrogenase is located intracellularly. After high‐speed centrifugation of cell‐free extracts, varying proportions, between 11 and 90%, of the hydrogenase were detected in the soluble fraction, the rest being associated with the membrane fraction.