Open AccessNutrition‐dependent modulations of protein synthesis in Candida albicans during germ‐tube formation or maintenance of the yeast form in N ‐acetyl glucosamine media
Author(s) -
Angiolella Letizia,
Torosantucci Antonella,
Carruba Giuseppe,
Cassone Antonio
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01701.x
Subject(s) - yeast , germ tube , candida albicans , glucosamine , chemistry , microbiology and biotechnology , wheat germ , biochemistry , biology
Stationary phase, yeast‐form cells of Candida albicans grown in glucose‐yeast extract medium were shifted to N ‐acetylglucosamine (GlcNAc) and/or glucose medium, and the pattern of protein synthesized under conditions of a progressive decrease in the rate of total protein synthesis was analyzed by SDS‐PAGE and autoradiography. Marked temporal modulations in the rate of synthesis of some cytoplasmic proteins were detected both in cells forming germ‐tubes (at 37°C) and in yeast cells (at 28°C). The major modulated components showed molecular weights of 63, 53, 48 and 34 kDa. These products could not be qualified as heat‐shock or heat‐stroke proteins, because analogous modulations were observed on shifting cells from 28°C to 37°C or from 28°C to 28°C. However, no marked modulations in the synthesis of specific proteins were detected when amino acids were added to the medium fostering germ‐tube formation under conditions of unimpaired overall rate of protein synthesis. It is suggested that the modulations observed in cells incubated in GlcNAc‐glucose medium could represent a response to a nutritional stress.