Open AccessDetection of ampicillin and tetracycline resistance genes in uropathogenic Escherichia coli strains by affinity‐based nucleic acid hybrid collection
Author(s) -
Syvänen AnnChristine,
Korpela Kai
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01700.x
Subject(s) - tetracycline , escherichia coli , nucleic acid , biology , gene , ampicillin , amp resistance , microbiology and biotechnology , nucleic acid thermodynamics , hybridization probe , dna , genetics , rna , antibiotics
Abstract Uropathogenic Escherichia coli strains were analyzed for the presence of the ampicillin (Ap) and tetracycline (Tc) resistance genes by a novel nucleic acid solution hybridization technique. In this method probe pairs subcloned from the resistance genes are utilized. DNA from single colonies of 20 E. coli strains was released and analyzed without purification by the 3 h hybridization test. The respective resistance genes were easily identified by the test.