Open AccessMannoproteins from the cell wall of Kluyveromyces lactis
Author(s) -
Herrero Enrique,
Boyd Alan
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01697.x
Subject(s) - kluyveromyces lactis , dithiothreitol , cell wall , saccharomyces cerevisiae , biochemistry , kluyveromyces , protoplast , antiserum , yeast , spheroplast , biology , chemistry , enzyme , escherichia coli , antibody , gene , immunology
Wall mannoproteins from Kluyveromyces lactis have been solubilised by treatment of cell walls with sodium dodecyl sulphate (SDS) or zymolyase. While the former reagent liberates a large number of molecular species, zymolyase preferentially releases a high‐molecular‐weight material that is sensitive to endo‐ β ‐ N ‐acetylglucosaminidase H, and a 29‐kDa molecule that reacts with the antiserum raised against a similar species from walls of Saccharomyces cerevisiae . In contrast with observations on isolated walls of S. cerevisiae , dithiothreitol pretreatment of K. lactis walls does not enhance the effect of zymolyase upon mannoprotein release. However, the action of thiol agents is still necessary to obtain protoplasts by zymolyase digestion from K. lactis whole cells.