Open AccessMapping of mutations affecting pyoverdine production in Pseudomonas aeruginosa
Author(s) -
Hohnadel Dany,
Haas Dieter,
Meyer JeanMarie
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01695.x
Subject(s) - pyoverdine , pseudomonas aeruginosa , siderophore , mutant , pseudomonadaceae , chemistry , pseudomonadales , microbiology and biotechnology , biology , gene , mutagenesis , biochemistry , bacteria , genetics
Pyoverdine, the yellow‐green fluorescent pigment produced by Pseudomonas aeruginosa , is a highly efficient siderophore. Pyoverdine‐deficient ( pvd ) mutants of P. aeruginosa PAO isolated after mutagenesis were non‐fluorescent and unable to grow in the presence of 2.8 mM ethylenediamine‐di‐( o ‐hydroxyphenylacetate) (EDDHA). Addition of purified pyoverdine to media containing EDDHA restored growth of pvd mutants. 6 pvd mutations were mapped between catA and mtu ‐9002 (at 65–70 min on the chromosome map) by R68.45‐mediated conjugation. 2 slightly leaky pvd mutations were localised between argC and strA (at 35 min) by transduction. Thus, we have identified at least 2 genes or gene clusters required for pyoverdine production in P. aeruginosa .