Open AccessMolecular cloning of the F8 fimbrial antigen from Escherichia coli
Author(s) -
Hacker Jörg,
Ott Manfred,
Schmidt Günter,
Hull Richard,
Goebel Werner
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01683.x
Subject(s) - biology , fimbria , polyclonal antibodies , antiserum , microbiology and biotechnology , escherichia coli , molecular cloning , cloning (programming) , gene , genetics , antigen , peptide sequence , computer science , programming language
The genetic determinant coding for the P‐specific F8 fimbriae was cloned from the chromosome of the Escherichia coli wild‐type strain 2980 (O18:K5:H5:F1C, F8). The F8 determinant was further subcloned into the Pst I site of pBR322 and a restriction map was established. In a Southern hybridization experiment identity between the chromosomally encoded F8 determinant of 2980 and its cloned counterpart was demonstrated. The cloned F8 fimbriae and those of the wild type strain consist of a protein subunit of nearly 20 kDa. F8 fimbriated strains were agglutinated by an F8 polyclonal antiserum, caused mannose‐resistant hemagglutination and attached to human uroepithelial cells. The cloned F8 determinant was well expressed in a variety of host strains.