Open AccessTime‐resolved fluorimetry in the study of attachment of staphylococci and streptococci on substrate‐bound fibronectin
Author(s) -
Kuusela Pentti,
Vartio Tapio,
Vuento Matti,
Myhre Erling B.,
Hemmilä Ilkka
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01666.x
Subject(s) - fibronectin , bacteria , microbiology and biotechnology , chemistry , adhesion , staphylococcus aureus , fluorescence spectroscopy , fluorescence , biochemistry , biology , genetics , organic chemistry , extracellular matrix , physics , quantum mechanics
The application of time‐resolved fluorimetry was evaluated in the study of staphylococcal and streptococcal attachment to fibronectin‐coated coverslips. The test system allowed the use of low bacterial concentrations (2 × 10 5 −10 7 bacteria per ml), in contrast to the much higher concentrations of bacteria used in earlier assays. The bacteria attached much better to fibronectin‐coated plastic surfaces than to albumin‐coated ones, but there were differences between the individual strains. Soluble fibronectin inhibited the adsorption of staphylococci but enhanced streptococcal attachment to fibronectin‐coated surfaces. Purified antibodies to fibronectin inhibited both staphylococcal and streptococcal adhesion in a dose‐dependent way. Our results show that time‐resolved fluorimetry is a very sensitive method for quantitating bacterial attachment.