Open AccessCloning and DNA homology of 3‐isopropylmalate dehydrogenase genes from thermophilic bacilli
Author(s) -
Sekiguchi T.,
Suda M.,
Sekiguchi T.,
Nosoh Y.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01663.x
Subject(s) - thermophile , thermus thermophilus , bacillus coagulans , bacillus subtilis , biology , thermus , escherichia coli , dna , gene , bacilli , methanococcus , microbiology and biotechnology , biochemistry , genetics , bacteria , enzyme
The genes coding for 3‐isopropylmalate dehydrogenase from the moderate thermophile Bacillus caldotenax and the extreme thermophile Bacillus caldolyticus were cloned in Escherichia coli using pBR322 as a vector. The restriction maps of the cloned fragments were determined and the small fragments carrying the enzyme gene were subcloned. DNA‐DNA hybridization of DNA fragments containing the gene from mesophiles ( Bacillus subtilis, E. coli and Saccharomyces cerevisiae ) and thermophiles (Bacillus coagulans, B. caldotenax, B. caldolyticus and Thermus thermophilus ) were examined. The DNA fragments from the thermophiles hybridized with each other, but not with the mesophile DNA fragments.