Open AccessUse of gene fusions to study the expression of fnr, the regulatory gene of anaerobic electron transfer in Escherichia coli
Author(s) -
Pascal MarieClaire,
Bonnefoy Violaine,
Fons Michel,
Chippaux Marc
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01662.x
Subject(s) - escherichia coli , gene , plasmid , gene product , biology , bacteriophage , lac operon , gene expression , fusion gene , anaerobic exercise , regulation of gene expression , microbiology and biotechnology , genetics , physiology
Fusions between fnr , the regulatory gene for anaerobic electron transfer, and lacZ were obtained by insertion of Mu d II1734 bacteriophage in the fnr gene cloned in a plasmid. After transfer onto the chromosome, study of the fusion showed that the expression of fnr is independent of anaerobiosis, negatively regulated by its own product and partly positively controlled by cyclic AMP.