Open AccessConstruction of an excretion vector: Extracellular production of Aeromonas xylanase and Bacillus cellulases by Escherichia coli
Author(s) -
Kato Chiaki,
Kobayashi Tetsuo,
Kudo Toshiaki,
Horikoshi Koki
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01661.x
Subject(s) - cellulase , escherichia coli , xylanase , microbiology and biotechnology , extracellular , chemistry , excretion , aeromonas , bacteria , biochemistry , biology , enzyme , gene , genetics
A new cloning vehicle, pEAP37 was constructed to develop the excretion system of Escherichia coli . This plasmid, derived from pEAP1, carried the penicillinase gene from an alkalophilic Bacillus sp. and the chloramphenicol acetyltransferase gene from pBR329 as selective markers. The Bacillus N‐4 cellulase gene, Bacillus 1139 cellulase gene and Aeromonas sp. 212 xylanase L gene was inserted into pEAP37, and the distribution of the plasmid‐encoded enzymes was analyzed. Most of these enzyme activities were found in the periplasmic space of E. coli when these extracellular enzyme genes were inserted into pBR322. On the other hand, most of these activities were observed in the culture medium when inserted into pEAP37.